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Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and <t>PCNA</t> to mark ves- sels and <t>proliferating</t> cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.
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Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Journal: Plastic & Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/prs.0000000000010753

Figure Lengend Snippet: Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Article Snippet: To detect the vessels and proliferating cells (early G1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Western Blot